NACHA requires that same-day items are posted by 5 PM local time the same day they are received. 

The statement “by 5:00 PM" means ‘anytime’ before 5:00 PM. That means 6:00 PM AT, 5:00 PM ET, 4:00 PM CT, 3:00 PM MT, and 2:00 PM PT, all of which are by 5:00 PM local time, are acceptable.

NACHA Funds Availability Requirements
As of August 2026; refer to https://www.nacha.org/system/files/2021-03/SDA_Schedules_and_Funds_Availability.pdf for the latest requirements.
 
Processing Window ODFI Deadline RDFI Receipt Time Settlement Credit Funds Availability Requirement
First Same Day ACH Window 10:30 a.m. ET 12 noon ET  1:00 p.m. ET  1:30 p.m. RDFI local time
Second Same Day ACH Window  2:45 p.m. ET  4:00 p.m. ET 5:00 p.m. ET 5:00 p.m. RDFI local time
Third Same Day ACH Window 4:45 p.m. ET 5:30 p.m. ET 6:00 p.m. ET End of processing day